Performance
1.3 Gbp) were processed to remove SMRT bell adapters, short and low-quality reads (< 80% accuracy) using SMRT Analysis version 2.3 ( A total of 155,828 filtered reads (average length, 8.9 Kb) were used for de novo assembly using Celera Assembler , with self-correction of the PacBio reads. A total of 1091 Mb paired-end Illumina reads screened from a 500-bp genomic library were mapped using SOAP to the bacterial plasmid database for tracing the presence of any plasmid and filling the gaps. The de novo assembly resulted in five contigs, representing the K. michiganensis BD177 complete genome of 6,812,698 bp with GC content % in a single chromosome and four plasmids. The annotated genome contains 6714 genes, 25rRNA genes, and 86 tRNA genes (Table 1).
16S rRNA phylogenetic data and phenotypic of remote filters BD177
Restriction parsimony mainly based and you will maximum likelihood phylogenetic trees using the sequence one to encodes into 16S rRNA gene indicated that the new nearest species so you can BD177 is K. michiganensis and K. oxytica (A lot more document step 1: Fig. S1, A lot more document 2: Dining table S1). Evolutionary divergence range of 16S rRNA gene sequence between BD177 and K. michiganensis E718 is minimum well worth (BD177 compared to K. michiganensis E718: 0.0079) certainly one of all sequences. Biochemical tests was in fact performed towards BD177 of the API20E to verify their status throughout the K. oxytoca and you will K. michiganensis. Biochemical functions off K. oxytoca ATCC13182(T)(=NCTC13727) and you may K. michiganensis W14(T)(= DSM2544) was indeed extracted from BacDive Webservices pared that have K.
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